Part:BBa_K4407013:Design
Plac-aceE，Expression of aceE in response to lactose
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 368
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 368
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 368
Illegal BglII site found at 428
Illegal BglII site found at 2277
Illegal BglII site found at 2685
Illegal BamHI site found at 1237 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 368
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 368
Illegal NgoMIV site found at 3307
Illegal AgeI site found at 2018 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
In our project, we used this device to construct the facultative anaerobic Clostridium tyrobutyricum (C. tyrobutyricum). aceE protein is a component of the PDH complex and catalyzes the conversion of pyruvate to acetyl-CoA and CO2. It can function well in aerobic environment, therefore allowing engineered C. tyrobutyricum to have an alternative metabolic pathway in the presence of oxygen. This device substitutes the main metabolic enzyme PFOR, which is inactivated under aerobic condition, with PDH in the presence of oxygen. In aerobic environment, we can use lactose to induce aceE expression in the recombinant C. tyrobutyricum to enhance its viability.
Source
Plac is based on BBa_K4119006. aceE is based on BBa_K4407002. Terminator is based on BBa_K3585002.